Smits Group

Group leader

Title

Name

First Name

Position

Dr.

Smits

Sander

Senior Researcher


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Researchers

Title

Name

First Name

Position


BioStruct Fellow

Title

Name

First Name

Position

PhD student


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Further Team Members

Technicians:

Master/Diploma students:

2

PHD/MD students:

2


Contact

Sander Smits
Membrane Transport Group
Institute of Biochemistry
Heinrich Heine University
Universitaetsstr. 1
40225 Duesseldorf

Tel : +49 211 81-12647
Fax: +49 211 81-15310

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Expertise in Structural Biology

  • X-ray crystallography

X-ray crystallography is the most used technique to determine the three-dimensional structures of biological molecules. The first step of X-ray crystallography is the crystallization of the protein.  To do so the protein needs to be purified in a homogenous state and also in one conformation. To achieve this biochemical analysis like, size exclusion chromatography studies or mutational studies are helpful to obtain an idea what the protein likes and what it disfavors. In crystals, protein molecules form a solid lattice, in which the individual building blocks are repeated in all three dimensions. Protein crystals scatter X-rays, which can be used to collect a diffraction pattern from which the structural information can be calculated. Thus, atomic information becomes available which is the prerequisite to understand the function of proteins/ enzymes with respect to catalysis or the role of disease-related mutations.

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Main areas of research interest

  • ABC-transporters
  • Lantibiotics/antimicrobial peptides
  • Protein kinetics
  • Protein modification
  • Transport across membranes

Facilities

The laboratories cover the following gene technology security levels:

S1

Other security laboratories or special facilities:

  • Radioactive laboratory

Laboratory Equipment

General equipment available in the laboratories:

  • Biochemistry
  • Biophysics
  • Molecular biology
  • Microbiology
  • Biotechnology
  • Biocomputing


Special equipment, which is not generally available in most laboratories:

  • X-ray Generator
  • Crystallization Robot
  • ITC Setup


Non-standard methods, which are established in the laboratory

  • In vitro translation
  • Incorporation of non-natural amino acids

Important References

  • announ, C., Norder, H. & Lindh, M. (2000)  An aberrabt genotype revealed in recombinant hepatitis B virus strains from Vietnam, J. Gen. Virol. 81,2267-2272.

Cooperators

  • Gohlke, Holger Prof., Heinrich Heine University, Duesseldorf, Germany
  • Bremer, Erhard Prof. University of Marburg, Germany
  • Jäger, Karl Prof., Heinrich Heine University, Duesseldorf, Germany
  • Driessen, Arnold Prof, University of Groningen, The Netherlands
  • Moll, Gert Dr., Biomade
Responsible for the content: E-MailBioStruct Office