Jose Group

Group leaders

Title

Name

First Name

Position

Prof. Dr.

Jose

Joachim

Head of the Institute

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Researchers

Title

Name

First Name

Position

Dr.

Maas

Ruth

Senior Scientist

Dr.

Sonnenberg

Nadine

Post Doc


BioStruct Fellow

Title

Name

First Name

Position

Dipl.Biol.

Gawarzewski

Iris

PhD student

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Further Team Members

Technicians:

2

PHD/MD students:

11


First Contact

Joachim Jose
Bioanalytics
Pharmaceutical and Medicinal Chemistry
Heinrich-Heine-University
Universitätsstr. 1, Geb 26.23 02, R40
40225 Düsseldorf
Germany

Tel : +49 211 81-13848
Fax: +49 211 81-13847


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Second Contact

Ruth Maas
Bioanalytics
Pharmaceutical and Medicinal Chemistry
Heinrich-Heine-University
Universitätsstr. 1, Geb 26.23 02, R40
40225 Düsseldorf
Germany

Tel : +49 211 81-12525
Fax: +49 211 81-13847
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Special Methodical Expertise

  • Surface Display of recombinant proteins in E. coli
  • Membrane protein expression and preparation
  • Biocatalytic synthesis by surface displayed enzymes
  • Development of bioanalytic assays

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Main areas of research interest

  • Evolutive drug design by surface display library screening
  • Biocatalytic synthesis of drug or building blocks by whole cell biocatalysts with surface displayed enzymes
  • Test assay development using whole cells of E. coli with surface displayed target proteins

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Facilities

The laboratories cover the following gene technology security levels:

S1

Laboratory Equipment

General equipment available in the laboratories:

  • Biochemistry
  • Molecular biology
  • Biotechnology
  • Microbiology


Special equipment, which is not generally available in most laboratories:

  • HPLC-MS, chiral HPLC, capillary electrophoresis, 2D-electrophoresis, elementary analysis, NMR, AAS, microplate fluorescence-, luminescence reader, HPTLC.


Non-standard methods, which are established in the laboratory:

  • Surface display library development
  • Outer membrane protein isolation

Important references

  • Jose J, Jähnig F, Meyer TF (1995) Common structural features of IgA1-protease-like outer membrane protein autotransporters. Mol Microbiol 18: 380-382.
  • Jose J, Betscheider D, Zangen D (2005) Bacterial Surface Display Library Screening by target enzyme labelling: Identification of New Human Cathepsin G Inhibitors. Anal Biochem, 346:258-267.
  • Jose J, Meyer TF (2007) The autodisplay story – from discovery to biotechnical and biomedical applications. Microbiol Mol Biol R, 71:600-619
  • Schultheiss E, Weiss S, Winterer E, Maas R, Heinzle E, Jose J (2008) Esterase autodisplay: enzyme engineering and whole cell activity determination by microplates with integrated pH-sensors. Appl Environ Microbiol, 74:4782-4791.
  • Jose J, Chung JW, Jeon JB, Maas RM, Nam CH, Pyun JC (2009) E. coli with autodisplayed Z-domain of protein A for signal amplification of SPR biosensensor. Biosens Bioelectron, 24: 1324-1329.

Cooperators

  • Pyun, Jae-Chul, Prof. Dr., Yonsei University, Seoul, Korea
  • LeBorgne, Marc, Prof. Dr., Université Claude Bernard, Lyon, France
  • Olgan, Sureyya, Prof. Dr., Ankara University, Ankara, Turkey
  • Bernhardt, Rita, Prof. Dr., Biochemistry, Saarland University, Saarbrücken, Germany
  • Meyer Thomas, Prof. Dr., MPI of Infection Biology, Berlin, Germany
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