Jaeger Group

Group leader

Title

Name

First Name

Position

Prof. Dr.

Jaeger

Karl-Erich

Head of the Institute

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Researchers

Title

Name

First Name

Position

Dr.

Krauss

Ulrich

Group leader Molecular Biophotonics

Prof. Dr.

Hummel

Werner

Group leader Biotransformations

Dr.

Drepper

Thomas

Group leader Bacterial Photobiotechnology

Dr.

Rosenau

Frank

Group leader Microbial Expression Systems

Dr.

Wilhelm

Susanne

Group leader Bacterial Autotransporters


BioStruct Fellow

Title

Name

First Name

Position

Msc.

Rani

Raj

PhD student

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Further Team Members

Technicians:

4

Master/Diploma students:

4

PHD/MD students:

26


First Contact

Institut für Molekulare Enzymtechnologie
Forschungszentrum Jülich
Stetternicher Forst
52426 Jülich
Germany

Tel : +49 2461 61-3716
Fax: +49 2461 61-2490
Molekulare Enzymtechnologie

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Second Contact

Molecular Biophotonics
Institut für Molekulare Enzymtechnologie
Forschungszentrum Jülich
Stetternicher Forst
52426 Jülich
Germany

Tel : +49 2461 61-2939
Fax: +49 2461 61-2490

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Special Methodical Expertise

  • enzyme gene identification – metagenomics
  • protein expression systems
  • large-scale (fermenter) protein production and purification
  • protein engineering and directed evolution
  • photochemistry of photoreceptor proteins

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Main areas of research interest

  • Biotechnological applications of photoreceptor proteins
  • Structure and function of flavin-binding photoreceptor proteins
  • Photobiology and physiology of non-photosynthetic bacteria

Facilities

The laboratories cover the following gene technology security levels:

S1, S2

The laboratories cover the following biohazard levels:

BSL1, BSL2

Laboratory Equipment

General equipment available in the laboratories:

  • Biochemistry
  • Biotechnology
  • Molecular biology
  • Microbiology
  • Imaging


Special equipment, which is not generally available in most laboratories:

  • Colony-picking and liquid-handling robot, automatic bioreactors (up to 15 Liter)


Non-standard methods, which are established in the laboratory:

  • Photochemistry of flavo-proteins
  • protein-engineering by rational design and by and directed evolution
  • high-throughtput screening technology to identify and characterize enzymatic activities and enantioselectivities

Important references

  • Drepper, T., T. Eggert, F. Circolone, A. Heck, U. Krauss, J. K. Guterl, M. ,Wendorff, A. Losi, W. Gärtner, and K. E. Jaeger. 2007. Reporter proteins for in vivo fluorescence without oxygen. Nat. Biotechnol 25:443-5.
  • Krauss, U., A. Losi, W. Gartner, K. E. Jaeger, and T. Eggert. 2005. Initial characterization of a blue-light sensing, phototropin-related protein from Pseudomonas putida: a paradigm for an extended LOV construct. Phys Chem Chem Phys 7:2804-11.
  • Becker, S., H. Hobenreich, A. Vogel, J. Knorr, S. Wilhelm, F. Rosenau, K. E. Jaeger, M. T. Reetz, and H. Kolmar. 2008. Single-cell high-throughput screening to identify enantioselective hydrolytic enzymes. Angew Chem Int Ed Engl 47:5085-8.
  • Wilhelm, S., F. Rosenau, S. Becker, S. Buest, S. Hausmann, H. Kolmar, and K. E. Jaeger. 2007. Functional cell-surface display of a lipase-specific chaperone. Chembiochem 8:55-60.
  • Tielker D, Eichhof I, Jaeger KE, Ernst JF. 2009 FMN-based fluorescent protein as an oxygen-independent reporter in Candida albicans and Saccharomyces cerevisiae. Eukaryot Cell, in press

Cooperators

  • Losi, Aba, Dr., Department of Physics-University of Parma, Parma, Italy
  • Gärtner, Wolfgang, Prof. Dr., Max-Planck-Institut für Bioanorganische Chemie, Mülheim, Germany
  • von Haeseler, Arndt, Prof. Dr., Center for Integrative Bioinformatics, Vienna, Austria
  • Benkovic, Stephen J., Prof. Dr., Department of Chemistry, Pennsylvania State University, USA
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