Bott / Brocker Group

Group leader

Title

Name

First Name

Position

Prof. Dr.

Bott

Michael

Head of the Institute

Dr.

Brocker

Melanie

Group leader

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Researchers

Title

Name

First Name

Position

Dr.

Polen

Tino

Group leader

Dr.

Eggeling

Lothar

Group leader

Dr.

Freudl

Roland

Group leader

Dr.

Bringer-Meyer

Stephanie

Group leader


BioStruct Fellow

Title

Name

First Name

Position

Dipl.Biol.

Schuplezow

Xenia

PhD student

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Further Team Members

Technicians:

9

Master/Diploma students:

5

PHD/MD students

20


First Contact

Melanie Brocker
Institute of Biotechnology 1
Forschungszentrum Jülich GmbH
Wilhelm-Johnen-Strasse
52428 Jülich
Germany

Tel  : +49 2461 61-5168
Fax: +49 2461 61-2710

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Second Contact

Michael Bott
Institute of biotechnology 1
Forschungszentrum Jülich GmbH
Wilhelm-Johnen-Strasse
52428 Jülich
Germany

Tel : +49 2461 61-3294
Fax: +49 2461 61-2710

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Special Methodical Expertise

  • Transcriptomics using DNA microarray technology
  • Proteomics including MALDI-TOF mass spectrometry
  • Metabolic engineering

Main areas of research interest

  • Elucidation of regulatory networks and mechanisms in biotechnologically relevant bacteria by studying transcriptional regulators, two-component signal transduction systems and serine/threonine protein kinases.
  • Analysis of the energy metabolism in biotechnologically relevant bacteria by studying the respiratory chain and ATP-generating reactions.
  • Development of microbial producer strains for amino acids, organic acids and other metabolites of industrial relevance using metabolic engineering

Facilities

The laboratories cover the following gene technology security levels:

S1

Other security laboratories or special facilities:

  • radioactive laboratory

Special methods

General equipment available in the laboratories:

  • Biochemistry
  • Molecular biology
  • Biotechnology
  • Microbiology


Special equipment, which is not generally available in most laboratories:

  • 2-D-gel equipment, fluorescence scanner (GenePix scanner for DNA microarrays and Typhoon scanner for proteomics), Spot-Picker, MALDI-TOF/TOF-MS, GC-MS, Biacore, ITC


Non-standard methods, which are established in the laboratory:

  • DNA microarray technology, 2D gel electrophoresis, protein identification and characterization by MALDI-TOF-MS, metabolite analysis by GC-MS, interaction studies using Biacore and isothermal titration calorimetry (ITC).

 

 

Important references

  • Kaspar, S., Perozzo, R., Reinelt, S., Meyer, M., Pfister, K., Scapozza, L., and Bott, M. (1999) The periplasmic domain of the histidine autokinase CitA functions as a highly specific citrate receptor. Mol. Microbiol. 33, 858-872.
  • Niebisch, A. and Bott, M. (2003). Purification of a cytochrome bc1-aa3 supercomplex with quinol oxidase activity from Corynebacterium glutamicum. Identification of a fourth subunit of cytochrome aa3 oxidase and mutational analysis of diheme cytochrome c1. J. Biol. Chem. 278: 4339-4346.
  • Wennerhold, J., Krug, A. and Bott, M. (2005). The AraC-type regulator RipA represses aconitase and other iron proteins from Corynebacterium under iron limitation and is itself repressed by DtxR. J. Biol. Chem. 280, 40500-40508
  • Bode, J. G., Schweigart, J. ,Kehrmann, J., Ehlting, C., Schaper, F., Heinrich, P.C. & Häussinger, D. (2003). TNF-alpha induces tyrosine phosphorylation and recruitment of the Src homology protein-tyrosine phosphatase 2 to the gp130 signal-transducing subunit of the IL-6 receptor complex. J Immunol 171,257-266.
  • Frunzke, J., Engels, V., Hasenbein, S., Gätgens, C. and Bott, M. (2008). Coordinated regulation of gluconate catabolism and glucose uptake in Corynebacterium glutamicum by two functionally equivalent transcriptional regulators, GntR1 and GntR2. Mol. Microbiol. 67, 305-322

Cooperators

  • Eikmanns, Bernd, Prof. Dr., University of Ulm, Germany
  • Bramkamp, Marc, Dr., University of Cologne, Germany
  • Wendisch, Volker, Prof. Dr., University of Münster, Germany
  • Burkovski, Andreas, Prof. Dr., University of Erlangen-Nürnberg, Germany
  • Pohl, Ehmke, Dr., University of Durham, U.K.
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