Ms. Claudia Beumer

 

 

 

 

 

 

 

Claudia Beumer M.Sc.

Heise Group
Institute for Structural Biology and Biophysics
Research Center Jülich GmbH
Wilhelm-Johnen-Straße
Building: 05.8v
Floor/Room: 2030
52425 Jülich
Phone +49 2461 61-8069

Mini Academic CV

University degrees:

First degree or intermediate examination:

  • Bachelor of Science in Chemical Biology, 2009, Technical University of Dortmund, Dortmund, Germany

Second degree and/or intermediate examination:

  • Master of Science in Chemical Biology, 2011, Technical University of Dortmund, Dortmund, Germany

Attended conferences:

  • Advanced School on Biological Solid-State NMR, 2012, Brno, Czech Republic, selected

  • Summer School on Spin-Hyperpolarization, 2012, Leiden, Netherlands, selected

Prices/Scholarships:

  • 2012, Grant for a Training School, European Cooperation in Science and Technology (COST)

BioStruct PhD project

DNP-enhanced solid-state NMR spectroscopy of HIV-related membrane proteins
HIV-1 utilizes the transmembrane protein CD4 as the main receptor for T-cell invasion. Following the invasion of the host cell, HIV-1 has developed multiple strategies to downregulate CD4 from the cell surface, since the continuous expression of the CD4 receptor may be detrimental to efficient viral replication and spread. One strategy for this CD4 downregulation is the proteasomal degradation of newly synthesized CD4 from the endoplasmic reticulum. This degradation is induced by the physical interaction of CD4 with the viral accessory protein Vpu, a membrane protein, which initiates a cascade of events leading to the degradation of CD4. We use solid-state NMR spectroscopy to study the interaction between Vpu and CD4 in liposomes. For this purpose, one aim of the project will be the expression and purification of the membrane proteins HIV-1 Vpu and human CD4 and their reconstitution into liposomes. Another part of the project will be the investigation of the interaction site of CD4 and Vpu in liposomes with signal-enhanced solid-state NMR spectroscopy, like Dynamic Nuclear Polarization (DNP), using differential isotopic labeling. Along with this, the sample preparation has to be optimized for DNP measurements.

Supervisors

Topic Supervisor:

undefined Prof. Dr. Henrike Heise, Institut für physikalische Biologie & Institut für Neurosciences and Biophysics (INB)/ Institute 2: Molecular Biophysics (INB-2), HHUD & Research Center Jülich GmbH, Heise Group

Complementary Supervisor:

undefinedProf. Dr. Claus A. M. Seidel, Molecular Physical Chemistry, HHU Düsseldorf

Third Supervisor:

PD Dr. Bernd König, ICS-6 / Structural Biochemistry, FZ Jülich

Responsible for the content: E-MailBioStruct Office