Mr. Sven Reimann

Photo of Sven  Reimann

Sven Reimann M.Sc.

nstitut für Biochemie
Group/Department: Lehrstuhl für Biochemie I, Arbeitsgruppe Membrantransport
Heinrich-Heine-Universität Düsseldorf
Universitätsstraße 1
Building: 26.42
Floor/Room: 02.12
40225 Düsseldorf
Phone +49 211 81-10774

Mini Academic CV

University degrees:

First degree or intermediate examination:

  • B.Sc. Biochemistry

Second degree and/or intermediate examination:

  • M.Sc. Biochemistry

Attended conferences:

  • Pelzer, A., Reimann, S., Laschinski, M., Rosenau, F., Jaeger, K.-E., Wilhelm, S. (2012). The novel subtilase SprP influences the lifestyle of Pseudomonas aeruginosa. Jahrestagung der Vereinigung für Allgemeine und Angewandte Mikrobiologie (VAAM), Tübingen

BioStruct PhD project

Investigation of the multicomponent Type 1 secretion system from E.coli
Institute of Biochemistry The type 1 secretion machinery of E.coli secretes the 100 kDa toxin HlyA in one step across both the inner and outer membrane. This machinery consists of three proteins: the inner membrane proteins HlyB and HlyD as well as the outer membrane protein TolC. HlyB belongs to the super family of ATP binding cassette transporters and the energy gained by ATP hydrolysis is used to translocate HlyA. Within my doctoral thesis two projects will be tackled. First the structure of HlyB will be investigated using X-ray crystallography. Here, initial crystals were obtained and need to be improved. A second goal is the purification of the fully assembled secretion complex. To achieve this a modified substrate will be used which locks the system in one distinct state, which allows the purification and subsequent characterization. Once achieved, a low-resolution structure of the whole complex will be solved using electron microscopy and single particle analysis.

Supervisors

Topic Supervisor:

undefinedProf. Dr. Sander Smits, Institute for Biochemistry, Smits Group

Complementary Supervisor:

undefinedProf. Dr. Lutz Schmitt, Institute for Biochemistry, Schmitt Group

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