Mr. Harish Chandra Thakur

 

 

 

 

 

 

Harish Chandra Thakur M.Sc.

Piekorz Group
Institute for Biochemistry and Molecular Biology II
Heinrich Heine University
Universitätsstr.1
Building: 22.03
40225 Düsseldorf
Phone +49 211 81-11305
Fax +49 211 81-12726

Mini Academic CV

University degrees:

First degree or intermediate examination:

  • B.Sc. (Agriculture), 2007, BHU, I.Ag.Sc., Varanasi, India

Second degree and/or intermediate examination:

  • M.Sc. Life Science, 2009, JNU, SLS, New Delhi, India

Further degrees and/or interemediate examinations:

  • Diploma in Italian Language, 2006, BHU, Dept. of Language, Varanasi, India

Attended conferences:

  • Training Course in “Methods in structural biology” and workshop on “Fluorescence-based spectroscopic and calorimetric applications in the analysis of biomolecular interactions“, AG Ahmadian, Institute of Biochemistry and Molecular Biology II, 2.-6. Nov. 2009
  • Workshop on Transferable Skills about "Presenting Science - comprehensive, competent and convincing", iGRAD, HHU Düsseldorf, 16.-17. Nov. 2009
  • Workshop on Transferable Skill about "Fundamentals of Project management", iGRAD, HHU, Düsseldorf, 11.-12. Dec. 2009
  • Biostruct Minisymposium on „Structural biology of amyloidogenic proteins“, 30. Nov. 2009

Prices/Scholarships:

  • 2008- Qualified in CSIR JRF examination 21 Dec 2008, standardized test for Junior research fellow and lectureship eligibility conducted by Human Resource Development Ministry, Govt. of India.
  • 2008- All India Rank 124 (No. of candidate appeared 13505, Percentile score-99.05, GATE score-507) in Graduate Aptitude Test in Engineering (GATE)-2008 (a standardized test conducted by the Indian Institutes of Science, Bangalore, India, zip-560012, for graduate studies in life sciences including biomedical engineering)
  • 2007-2009 -Merit cum Means fellowship from Jawaharlal Nehru University New Delhi, For Masters.
  • 2004-2007 -Fellowship from District Welfare Society, Madhubani, Bihar, during my B.Sc.(Ag.) program

BioStruct PhD project

Structural and functional characterization of the centrosomal TACC3-chTOG protein complex, a new mitotic cancer target
The mitotic spindle apparatus consists of centrosomes, kinetochores and microtubules (MT). Together with various MT-associated proteins these structures are critically involved in the regulation of MT dynamics and faithful separation of chromosomes during mitosis. This process is tightly regulated during the cell cycle. Alterations in centrosomal and mitotic spindle architecture lead to chromosomal instability and aneuploidy with profound consequences for cell cycle progression and cellular survival. The mitotic spindle apparatus is often altered in pathologies from neurological diseases to neoplasia and represents a major cellular target for antitumor therapy. Transforming acidic coiled-coil (TACC) proteins have been identified as important structural components of the centrosome/spindle apparatus. These protein family shares a ~200 amino acid C-terminal coiled coil motif (CC) with only limited homology at the N-terminus. TACCs interact through their CC motif with the C-terminus of the MT polymerase chTOG which binds to MT ends through multiple N-terminal TOG domains. Depletion of TACC3 or chTOG interferes with centrosome integrity, centrosome-dependent assembly of MTs and spindle stability thereby leading to mitotic cell death or p53-dependent postmitotic cell cycle arrest. The major aim of my project is the determination and analysis of the three-dimensional structure of full length murine TACC3 and its CC domain alone and in complex with the C-terminus of murine chTOG as well as the in-depth biochemical analysis of such a bimolecular interaction. These analysis will provide (1) novel molecular insight in the interaction of TACC3 with the MT polymerase chTOG and (2) the opportunity to design inhibitors to block TACC3 binding to chTOG as candidate antineoplastic approach.

Supervisors

Topic Supervisor:

undefinedDr. Roland Piekorz, Institute for Biochemistry and Molecular Biology II, Heinrich Heine University Duesseldorf, Cellular signaling and mitosis group

Complementary Supervisor:

undefinedPD Dr. Reza Ahmadian, Institute of Biochemistry and Molecular Biology II, Heinrich Heine University Duesseldorf, Ahmadian Group

Complementary Supervisor:

undefinedProf. Dr. Lutz Schmitt, Institute of Biochemistry, Heinrich Heine University Duesseldorf, Schmitt Group

Responsible for the content: E-MailBioStruct Office