2nd BioStruct MasterClass 2011 "Macromolecular Interactions"

Pictures of Biostruct Masterclass 2011


BioStruct’s MasterClass 2011was organized by the PhD-Fellows of NRW Research School BioStruct. This meeting will bring together doctoral researchers from diverse backgrounds with renowned scientists in the area of structural biology research.

The NRW Research School BioStruct invited early stage researchers from the field of Structural Biology and related research areas to the 2nd ‘BioStruct’s MasterClass 2011’, at:


Heinrich Heine Universität, Düsseldorf

September 12th-15th, 2011

Building 26.41.00, lecture hall 6G


Renowed speakers provided a deep insight into the interactions of biological macromolecules and their examination via complex structural biology techniques. We additioanlly invited doctoral and postdoctoral researchers to give 15 minute oral presentation or poster presentation based on their research work.

On Wednesday September 14th the 2001 annual meeting of the bio-N3MR Network NRW was included in this year's BioStruct MasterClass:

link_bio_nmr link

During the Master Class 2011 there was time for networking in addition to scientific presentations, discussions and poster presentations. For example, a visit to the famous old town of the state capital Düsseldorf and a barbecue in a relaxed atmosphere at the botanical garden of the Heinrich-Heine-University were planned.

We enjoyed to welcome you at the MasterClass. More information can be found at www.biostruct.de.

Monday, September 12th, 2011

10:00 Registration in the foyer of lecture hall 6J, building 26.41.00
11:00 Welcome by Prof. Dr. Dr. H. Michael Piper, President of the Heinrich-Heine University Düsseldorf
Welcome by Prof. Dr. Detlev Riesner, Chairman of the Entrepreneur Foundation for the Advancement of Science and Young Researchers, HHU
Welcome by Lutz Schmitt, Chairman of theNRW Research School BioStruct
11:15 - 12:15 2 Short-Talks of doctoral researchers:
11:15 Thomas Classen, BioStruct, AG Pietruszka:
"Multicopper oxidase CueO from Rhodococcus erythropolis"
11:40 Jendrik Marbach, BioStruct, AG Birkmann:
"Enroute to expression and structural analysis of Prion Protein in Pichia pastoris"
12:15 - 13:15 Lunch
13:15 - 15:00 Peter Cherepanov, "The mechanism of HIV integration and its inhibition by strand transfer inhibitors: lessons from X-ray crystallography studies using a convenient model system"
15:00 - 15:15 short break
15:15 - 16:15 3 Short-Talks of doctoral researchers:
15:15 Leonid Rapatskiy, BioStruct, AG Lubitz "Detection of water binding sites in the oxygen evolving complex of Photosystem II poised in the S2 state using high field (94 GHz) 17O-ELDOR-detected NMR spectroscopy"
15:35 Iris Gawarzewski, BioStruct, AG Schmitt "Purification and crystallization of the His-tagged AIDA-I ß-barrel in the outer membrane of E. coli"
15:55 Imadeldin Elfaki, Duisburg-Essen "Analysis of Parvulin14/17"
16:15 - 16:45 Tea/Coffee break
16:45 - 18:30 Ulrich Benjamin Kaupp, ‘An ion channel that encodes single-molecule sensitivity in sperm' [Ion channels]

Tuesday, September 13th, 2011

09:00 - 10:45 Christine Ziegler, ‘Monitoring conformational changes in secondary
transporter activation by 2Dand 3D crystallization'[Electron microscopy and X-ray]
10:45 - 11:15 Tea/Coffee break
11:15 - 12:15 Poster session (odd numbers)
12:15 - 13:15 Lunch
13:15 - 15:00 Oliver P. Ernst, ‘Ligand interaction: Light induced conformational changes'
15:00 - 15:30 Tea/Coffee break
15:30 - 17:15 Crina Nimigean, ‘'Investigating ion channel gating and selectivity with structural techniques!
[Multiple strategies for structure determination: X-ray, NMR and EM]
18:30 Guided City Tour through Düsseldorf's Old Town 'The Brewery Path' Düsseldorf Altstadt
20:00 Congress Dinner at Old Town Restaurant "Brauerei Zum Schiffchen" for Speakers and registered participants
Restaurant "Brauerei zum Schiffchen"

Wednesday, September 14th, 2011

  Today including the 2011 annual meeting of Bio-N³MR Network NRW
09:00 - 10:45 Martin Blackledge, ‘Exploring Multiple Timescale Motions in Folded and Intrinsically Disordered Proteins using NMR'
[Liquid state NMR]
10:45 - 11:15 Tea/Coffee break
11:15 - 12:15 3 Short-Talks of doctoral researchers:
11:15 Peixiang Ma, Jülich:“Characterization of membrane anchored GABARAP using nanodiscs”
11:35 Sameer K. Singh, Jülich:“Solution NMR study of the interaction between human CD4 and HIV-1 VpU”
11:55 Sabu Varghese, Düsseldorf:
"Solid state NMR studies of the membrane binding heme protein nitrophorin 7 from Rhodnius prolixus"
12:15 - 13:15 Lunch
13:15 - 14:00 Poster session (even numbers)
14:00 - 15:45 Hartmut Oschkinat, ‘Structure determination of proteins in a native environment by solid state NMR'
[Solid state NMR]
15:45 - 16:15 Tea/Coffee break
16:15 - 18:00 Knud Nierhaus, ‘3D-Structure and Function: The tricks of the ribosome to be fast and precise”
19:00 BBQ at the Botanical Garden for attendees with previous notice Botanical Garden

Thursday, September 15th, 2011

09:00 - 10:45 Jakob Piehler, ‘Diffusion and interaction in type I interferon signalling'
[Ligand interaction]
10:45 - 11:15 Tea/Coffee break
11:15 - 12:15 3 Short-Talks of doctoral researchers:
11:15 Harish Chandra Thakur, BioStruct, AG Piekorz:
"Biophysical, biochemical, and functional characterization of the centrosomal protein TACC3"
11:35 Simon Sindbert, BioStruct, AG Seidel:
"Structure determination of an RNA four-way junction via Super Resolution FRET (SuRF)"
11:55 Arpita Roychoudhury, BioStruct, AG Häussinger/Oesterhelt:
"Observing the protecting effect of organic osmolytes in action at the single molecule level"
12:15 - 13:15 Lunch
13:15 - 15:00 Matthias Rief, ‘Exact title to be announced
15:00 - 15:30 Best Poster Award and Closure by Prof. Dr. Dieter Willbold,Chairman of NRW Research School BioStruct


Martin Blackledge is group leader at the Institute de Biologie Structurale Jean-Pierre Ebel (IBS) in Grenoble, France. He is an expert on liquid state Nuclear Magnetic Resonance (NMR) spectroscopy of proteins. Key research interests of his "Protein Dynamics and Flexibility by NMR" group are conformational flexibility of proteins in their different forms and the complex relationship between molecular motion and biological function and malfunction. His research spans from high resolution studies of the conformational energy landscape of folded proteins to the highly flexible behavior of intrinsically unfolded proteins.

Peter Cherepanov Peter Cherepanov is a Reader in Virology at Imperial College London, UK. The main focus of his research is the mechanism of the retroviral DNA integration machinery and its interactions with the host cell.

Knud H. Nierhaus is group leader at the Max-Planck-Institut für Molekulare Genetik. His group is interested in various aspects of ribosomes such as the mechanistics of assembly of bacterial ribosomes. Current examples are: (i) The functional interplay of the three tRNA-binding sites on the ribosome, the A, P and E sites, in the course of protein synthesis. (i) The importance of universal but non-canonical factors of protein synthesis such as EF4 and YbeB. Further aspects are inhibition mechanisms of antibiotics and preparations of functional complexes, the structure of which is analyzed in cooperation with Christian M. T. Spahn at the Charité, Berlin.

Crina Nimigean is Assistant Professor of Physiology and Biophysics in Anesthesiology at the Weill Cornell Medical College in New York. Her research is multi-disciplinary, combining structural and functional approaches to address fundamental mechanistic questions about the properties of ion channels. The current primary focus is on investigating gating, permeation, selectivity, modulation by ligands as well as interactions with putative accessory proteins in prokaryotic potassium channels such as KcsA, MthK, and MloK1.

Jacob Piehler is Professor of Biophysics and Managing director of the Center of Advanced Light Microscopy at the Department of Biology of the University of Osnabrück. His interests are protein-protein interactions involved in cytokine receptor assembly and signalling. These interactions are reconstituted in vitro using solid-supported membranes and explored by multiparameter solid phase detection and single molecule imaging techniques. Diffusion and assembly of signalling complexes are also explored in life cells by single molecule imaging techniques. For these purposes, biofunctional surface architectures and posttranslational labelling techniques are developed.

Matthias Rief Matthias Rief is a full professor of Biophysics at Technische Universität München. During his career he has developed and applied single molecule spectroscopy techniques like AFM and optical tweezers. He is interested in understanding the mechanics of molecular motors and the fundamental role played by the energy landscape of a biomolecule in response to tension.

Christine Ziegler Research in the group of Dr. Christine Ziegler at the MPI of Biophysics in Frankfurt focuses on stress response mechanisms in both prokaryotes (including pathogens) and eukaryotes. Her expertise is on the investigation of structure and function of membrane transporters and other proteins involved in osmotic stress response using a broad spectrum of different methods ranging from molecular biology and microbiology to structural biology and biophysical techniques. The most advanced project in the Ziegler's lab is the molecular mechanism of osmosensing and regulation in the Na+/ betaine transporter BetP from Corynebacterium glutamicum that is a member of the BCCT- family. The structure of BetP was solved in C. Ziegler's group by X-ray crystallography and electron crystallography of 2D crystals.

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